Idea of Rapid Preparation of Fatty Acid Methyl Ester Using In Situ Derivatization from Fresh Horse Mussel
نویسندگان
چکیده
The analysis of the fatty acid (FA) profile requires multiple preparation steps, which are lipid extraction followed by derivatization FA into a methyl ester (FAME). procedures time-consuming, and generally require large volumes sample sizes solvents. This report proposes technique for FAME from fresh horse mussels without step extraction. A rapid in situ using N,N-dimethylformamide dimethyl acetal (DMF-DMA) methylation alkali-transesterification was examined. In this method, acylglycerols free acids (medium to long-chain FA) targeted convert FAME. Direct gave only 58.7% with 12.4% triglyceride 21.1% FFA. alkali method showed low conversion efficiency due initial containing high contents moisture FFA (75.11% 14.3% total oil, respectively). reaction developed two steps derivatization. 50 mg methylated 1 mL DMF-DMA (100 °C, 15 min), transesterified 10 1% (w/v) NaOH methanol (60 3 min). monitored size-exclusion HPLC evaporative light-scattering detection. column 100 Å Phenogel toluene 0.25% acetic as mobile phase. yield 79.9% 7.8% 8.5% obtained. promising result higher lower It is simple (less than 20 min) that volume solvent preparation. However, increasing well variety samples should be further studied.
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ژورنال
عنوان ژورنال: Engineering proceedings
سال: 2021
ISSN: ['2673-4591']
DOI: https://doi.org/10.3390/asec2021-11142